Research Interests:

Using solid state NMR techniques to study the interactions between the membrane protein phospholamban and Ca 2+-ATPase.

Education:

2007-present	Postdoctoral Associate, University of Minnesota Twin Cities Dr. Gianluigi Veglia, Advisor
2003-2007	Ph.D. Chemistry, University of Minnesota Twin Cities Dr. Gianluigi Veglia, Advisor
1999-2003	B.S., Biochemistry/Molecular Biology B.S., Chemistry B.A., Political Science, University of Minnesota Duluth

Research Positions:

2007-present	Postdoctoral Associate University of Minnesota Twin Cities Department of Biochemistry, Molecular Biology, and Biophysics
2003-2007	Graduate Research Assistant University of Minnesota Department of Chemistry
2002-2003	Undergraduate Research Assistant University of Minnesota Duluth Dr. Cecelia Giulivi, Advisor

Teaching Positions:

2004	Teaching Assistant Physical Chemistry Laboratory, University of Minnesota
2003-2004	Teaching Assistant General Chemistry, University of Minnesota
2003	Teaching Assistant Biochemistry, University of Minnesota Duluth
2002	Teaching Assistant General Chemistry, University of Minnesota Duluth

Awards:

2005-2007	American Heart Association Pre-Doctoral Fellowship
2004-2005	National Institute of Health Neuro-Physical-Computational Sciences Grant
2003-2004	Kolthoff Fellowship (declined)
2003	Graduated Magna Cum Laude in Chemistry, Biochemistry/Molecular Biology and Political Science
2003	Graduated with Honors from the Departments of Chemistry and Political Science
2003	American Insitute of Chemist's Award for outstanting graduating senior in Chemistry and Biochemistry/Molecular Biology
2003	Francis de Groat award in Political Science for graduating honor student with the highest GPA
2001-2003	Recipient of four Undergraduate Research Opportunity Program awards
2001-2003	Awarded McGuire scholarship for high scholastic achievement in Chemistry
2002	Recipient of Summer Undergraduate Research Opportunities Program award
2002	Awarded Enbridge Energy scholarship for achievements in science and engineering
2000-2001	Awarded Iron Range scholarship for academic achievement college freshman year
2001-2002	Awarded Hypercube scholar given for achievement in physical chemistry course
1999-2003	Made Dean's list (>3.5 GPA) all eight semesters
1999-2003	Presidential scholarship for scholastic achievements in high school

 

Publications:

Deng, Y., Wang, Y., Holtz, B., Li, J., Traaseth, N.J.,Veglia, G., Stottrup, B.J., Elde, R., Pei, D., Guo, A., Zhu, X.Y., Fluidic and Air-stable Supported Lipid Bilayer and Cell-mimicking Microarrays, J. Am. Chem. Soc., 2008, 130, 19, 6267-71.

Traaseth, N.J., Verardi, R., Veglia, G., Asymmetric Methyl Group Labeling as a Probe of Membrane Protein Homo-oligomers by NMR Spectroscopy, J. Am. Chem. Soc., 2008, 130, 8, 2400-01.

Masterson, L.R., Mascioni, A., Traaseth, N.J., Taylor, S.S., Veglia, G., Allosteric Cooperativitiy in Protein Kinase A, PNAS, 2008, 105, 2, 506-11.

Traaseth, N.J., Ha, K.N., Verardi, R., Shi, L., Buffy, J.J, Masterson, L.R., Veglia, G., Structural and Dynamic Basis of Phospholamban and Sarcolipin Inhibition of Ca(2+)-ATPase, Biochemistry, 2008, 47, 1, 3-13.

Ha, K.N., Traaseth, N.J., Verardi, R., Zamoon, J., Cembran, A., Karim, C.B., Thomas, D.D., Veglia, G., Controlling the Inhibition of the Sarcoplasmic Ca2+-ATPase by Tuning Phospholamban Structural Dynamics,J. Biol. Chem., 2007, 282, 51, 37205-14.

Traaseth, N.J., Verardi, R., Torgersen, K.D., Karim, C.B., Thomas, D.D., Veglia, G., Spectroscopic Validation of the Pentameric Structure of Phospholamban, PNAS, 2007, 104, 37, 14676-81.

Gor'Kov, P.L., Chekmenev, E.Y., Li, C., Cotten, M., Buffy, J.J., Traaseth, N.T., Veglia, G., Brey, W.W., Using Low-E Resonators to Reduce RF Heating in Biological Samples For Static Solid-State NMR up to 900 MH, J. Mag. Res., 2007, 185, 77-93.

Traaseth, N.J., Buffy, J.J, Zamoon, J., Veglia G., Structural Dynamics and Topology of Phospholamban in Oriented Lipid Bilayers Using Multi-dimensional Solid-State NMR, Biochemistry, 2006, 45, 13827-34.

Buffy, J.J., Traaseth, N.J., Mascioni, A., Gor'kov, P.L., Chekmenev, E.Y., Brey, W.W., and Veglia, G., Two-Dimensional Solid-State NMR Reveals Two Topologies of Sarcolipin in Oriented Lipid Bilayers, Biochemistry, 2006, 45, 10939-46.

Traaseth, N.J., Thomas, D.D., and Veglia, G., Effects of Ser16 Phosphorylation on the Allosteric Transitions of Phospholamban/Ca²⁺-ATPase Complex , J. Mol. Biol., 358, 4, 2006, 1041-1050.

Buffy, J.J., Buck-Koehntop, B.A., Porcelli, F., Traaseth, N.J., Thomas, D.D., Veglia, G., Defining the Intramembrane Binding Mechanism of Sarcolipin to Calcium ATPase Using Solution NMR Spectroscopy, J. Mol. Biol., 358, 2, 2006, 420-429.

Metcalfe, E.E., Traaseth, N.J., Veglia, G., Serine 16 Phosphorylation Induces an Order-to-Disorder Transition in Monomeric Phospholamban, Biochemistry, 2005, 44, 11, 4386-96.

Haynes V, Elfering S, Traaseth NJ, Giulivi C Mitochondrial nitric-oxide synthase: enzyme expression, characterization, and reguluation. J. Bioenerg. Biomembr., 2004, 36, 341-6.

Traaseth NJ, Elfering S, Solien J, Haynes V, Giulivi C Role of calcium signaling in the activation of mitochondrial nitric-oxide synthase and citric acid cycle. Biochim. Biophys. Acta., 2004, 1658, 64-71.

Elfering S, Haynes V, Traaseth N, Ettl A, Giulivi C Aspects, mechanisms, and biological relevance of mitochondrial protein nitration sustained by mitochondrial nitric oxide synthase. Am. J. Physiol. Heart Circ. Physiol., 2004, 286, H22-9.

Haynes V, Elfering SL, Squires RJ, Traaseth NJ Solien J, Ettl A, Giulivi C Mitochondrial nitric-oxide synthase: role in pathophysiology. IUBMB Life, 2004, 55, 599-603.

Giulivi C, Traaseth NJ, Davies KJA Tyrosine oxidation products: analysis and biological relevance. Amino Acids, 2003, 25, 227-32.

Steffen M, Sarkela TM, Gybina AA, Steel TW, Traaseth NJ, Kuehl D, Giulivi C Metabolism of S-nitrosoglutathione in intact mitochondria. J. Biochem., 2001, 356, 395-402

Presentations:

Structure, Dynamics, and Interactions between Membrane Proteins by Solid-State NMR Spectroscopy. University of Minnesota-Minneapolis. 5th Annual Graduate Student Research Symposium, Department of Chemistry, May 16, 2006.

Tyrosine Oxidative Stress Products in Rat F1 and Submitochondrial Particles. University of Minnesota-Duluth, 2003.

 

Posters:

Coupled Motions in Protein Kinase A as Characterized by NMR. Masterson LR, Traaseth NJ, Mascioni A, Taylor SS, Veglia G. Experimental Nuclear Magnetic Resonance Conference, Pacific Grove, Ca, April 23-28, 2006.

Membrane Topology and Ca-ATPase Interaction of the Membrane Protein Sarcolipin. Buffy JJ, Traaseth NJ, Gor’kov PL, Chekmenev EY, Brey WW, Thomas DD, Veglia G. Experimental Nuclear Magnetic Resonance Conference, Pacific Grove, Ca, April 23-28, 2006.

Structural Dynamics and Topology of Phospholamban in Lipid Bilayers using Multi-dimensional Solid-State NMR. Traaseth NJ, Buffy JJ, Veglia G. Experimental Nuclear Magnetic Resonance Conference, Pacific Grove, Ca, April 23-28, 2006.

Applications of Low-E Static Probes to High Frequency Membrane Protein NMR. Gor’kov PL, Chekmenev EY, Cotton M, Buffy JJ, Veglia G, Traaseth NJ, Cross TA, Brey WW. Experimental Nuclear Magnetic Resonance Conference, Pacific Grove, Ca, April 23-28, 2006.

Probing the Regulation of Ca-ATPase by Phospholamban in Lipid Environments Using Solution and Solid-State NMR. Traaseth NJ, Buffy JJ, Zamoon J, Thomas DD, Veglia G. Keystone Symposia (Frontiers in Structural Biology), Keystone, CO, January 29 – February 3, 2006.

NMR Elucidation of the Binding Interface of Ca-ATPase with the Membrane Protein Sarcolipin. Buffy JJ, Traaseth NJ, Buck-Koehntop BA, Porcelli F, Thomas DD, Veglia G. Keystone Symposia (Frontiers in Structural Biology), Keystone, CO, January 29 – February 3, 2006.

Structural and Functional Dynamics of the Membrane Protein Phospholamban and its Mutants in Interaction with Ca-ATPase. Ha K, Traaseth NJ, Zamoon J, Veglia G. Keystone Symposia (Frontiers in Structural Biology), Keystone, CO, January 29 – February 3, 2006.

Defining Allosteric Regulation in Protein Kinase A. Masterson LR, Mascioni A, Traaseth NJ, Taylor SS, Veglia G. Keystone Symposia (Frontiers in Structural Biology), Keystone, CO, January 29 – February 3, 2006.

Topological and Structural Analysis of Phospholamban/Ca-ATPase Complex in Lipid Membranes Using Solid-State NMR. Traaseth NJ, Buffy JJ, Thomas DD, Veglia G. Pacifichem (International Chemical Congress of Pacific Basin Societies), Honolulu, HI, December 15-20, 2005.

Solid State NMR Elucidation of the Topology and Ca-ATPase Interaction of the Membrane Protein Sarcolipin. Buffy JJ, Traaseth NJ, Thomas DD, Veglia G. Pacifichem (International Chemical Congress of Pacific Basin Societies), Honolulu, HI, December 15-20, 2005.

Characterization of Phospholamban/Ca2+ATPase Interaction. Traaseth NJ, Buffy J, Veglia G. Experimental Nuclear Magnetic Resonance Conference, Providence, RI, April 10-15, 2005

Identification and Quantification of Tyrosine Oxidative Stress Products in Mitochondria. Traaseth NJ, Giulivi C. Sigma Xi Conference, University of Minnesota-Duluth, July 2002.

Metabolism of S-nitrosoglutathione in intact mitochondria. Gybina AA, Traaseth NJ, Giulivi C. Minnesota Academy of Sciences, University of St. Thomas, St. Paul, MN, May 2001.